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Fixed-Time
Insemination Of Beef Cattle With CIDR-Based Protocols
Results from the New Liskeard Agricultural Research Station
- Tom Hamilton,
Beef Systems Program Lead
Ontario Ministry of Agriculture, Food and Rural Affairs
- New Liskeard,
Ontario, Canada
-
Experiments conducted
during 1995 – 1998 in the Beef Program of the New Liskeard
Agricultural Research Station (OMAFRA/University of Guelph) examined
various methods of fixed-time, single insemination synchronized
breeding. Cattle were either large frame crosses (Char/Lim/Sim) or Ang X
large frame crosses. These trials involved a total of 488 cows and 252
heifers, inseminated in either a spring (May) or fall (Sept) breeding
period in each year. All cattle within a season were A.I.’d once, on a
group basis one day. Two experienced technicians (Gencor, Earlton)
provided A.I. services.
Strategies which
incorporated a CIDR-B® (Controlled Intravaginal Drug Release) device
were found to result in pregnancy rates which compared very favourably
with other fixed-time methods. The device contains progesterone, which
is released into the vagina during an insertion period of several days.
In these experiments, various combinations of other hormones were
administered with the CIDRs, with the objective of tightly synchronizing
ovulation. ®(CIDR-B, Vetrepharm Canada, InterAg New Zealand)
Table 1 shows
pregnancy rates by season and animal age class, for fixed-time
artificial insemination with CIDR-based protocols. To give an overall
yearly pregnancy rate, results from all protocols within a given
season were pooled. These results were then averaged across years
to give an overall outcome. In addition to the table, individual
protocols within a specific year and season gave fixed-time pregnancy
rates, which ranged from 60% to 95% for cows, and from 42% to 93% for
heifers.
Table 1:
Percent Pregnant to Fixed-time AI CIDR-based
Protocols,
Four-Year Averages and Ranges, NLARS
|
Breeding
Season
|
| |
Spring
|
Fall
|
|
Cows
|
76
(69 – 82)
|
70
(61 – 84)
|
|
Heifers
|
78
(67 – 88)
|
75
(50 – 90)
|
Based on the results from
these trials, the most effective protocol involved handling cattle 4X prior to
breeding (Figure 1, Treatment 1). The injection of progesterone on day 0, (in
addition to that from the CIDR), was found to be beneficial in this research. An
alternative protocol (Treatment 2), which requires handling 3X prior to
breeding, may give similar results, but further study is required to confirm
this.
Many animal factors affect
the success of synchronized breeding programs. Among these are overall health
status; length of the postpartum period (cows); % of mature body weight
(heifers); degree of body condition; adequate energy, protein, vitamin and
mineral nutrition; and handling stress. Good handling facilities are needed for
efficient processing and to reduce stress on both animals and people.
In these trials, cow
groups averaged 62 – 78 days postpartum at breeding, while heifer groups
averaged 14.1 to 14.6 months of age at breeding. Overall nutrition was excellent
and body condition scores were within the desired range. As well, animals were
familiar with the handling facilities and techniques employed.
Figure 1. CIDR –
Based Synchronization Protocols
| Trt |
- Day 1
- 8:00 a.m.
|
- Day 6
- 9:00 a.m.
|
- Day 7
- 9:00 a.m.
|
- Day 8
- 10:00 a.m.
|
- Day 9
- 1:00
p.m.
|
- 1
|
- -CIDR¹
inserted
- -Estradiol²
- -Progesterone³
|
- -Prostaglandin4
|
- -CIDR
removed
|
- -Estradiol²
|
-
AI
|
- 2
|
- -CIDR¹
inserted
- -Estradiol²
-Progesterone³
|
- --------
|
- -CIDR
removed
- -Prostaglandin4
|
- -Estradiol²
|
- AI
|
- ¹Controlled
Intravaginal Drug Release device, 1.9 grams progesterone
- ²Estradiol Bensoate, 1
milligram, intramuscular
- ³Progesterone, 100
milligrams, intramuscular
- 4
Prostaglandin,
25 milligrams of PGF2~ , intramuscular
Notes:
- The CIDR device has
Canadian approval for use with prostaglandin to synchronize estrus for
breeding on observed heat. CIDRs are available only through Veterinarians. The
addition of other drugs to synchronize ovulation for fixed-time
breeding must be done under Veterinary prescription. Consult your veterinarian
prior to the breeding season to establish a program suitable for your herd.
- Loss of CIDRs after
insertion ranged from 5% to 8%. To minimize losses from heifers, the tab may
be trimmed to leave about 4 inches protruding from the vulva.
- Timing of all procedures
is critical. The optimum window for breeding is 50 – 52 hours after CIDR
removal. There should be a minimum of 24 hrs between CIDR removal on day 7 and
estradiol injection on day 8.
- For Day 0, progesterone
and estradiol may be combined. This mixture must supply a total of 1 milligram
(mg) of estradiol benzoate and 100 milligrams (mg) of progesterone per
injection.
- With large numbers of
cattle (>40hd), separate animals into groups of approximately 20 hd, and
process the groups in the same sequence on each treatment day. This will
minimize the variation in timing between procedures for individual animals. A
team of two AI technicians will be more efficient than one, especially if
multiple sires are used.
- For additional
information on CIDRs and this research see www.vetrepharm.com
and www.aps.uoguelph.ca/beefupdate
. For additional information on cattle reproduction in general, see www.gov.on.ca/OMAFRA/english/livestock
.
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